TABLE 2.

Analysis of mtr2 mutant alleles

Genotype	mRNA export defecta	Location of:		Complemention by MEX67d	Synthetic lethality with nup85Δe	Complemention by NUP85f
		Mtr2pb	Mex67pc
MTR2	−	NPC	NPC
mtr2-9	+	Cytoplasm	Cytoplasm	−	+	+
mtr2-26	+	ND	Cytoplasm	−	+	+
mtr2-21	+	NPC	Cytoplasm	+	−	−

^aAnalyzed after 30 min at 37°C. −, no defect; +, defect. 

^bWild-type or mutant Mtr2p was localized as a fusion protein with GFP after 16 min at 36°C. ND, not determined. 

^cWild-type Mex67p was localized as a fusion protein with GFP after 16 min at 36°C. 

^dAnalyzed at 37°C. −, no complementation; +, complementation. 

^e−, no synthetic lethality; +, synthetic lethality. 

^fAnalyzed at 33°C. −, no complementation; +, complementation.