. 2024 Mar 11;20:99. doi: 10.1186/s12917-024-03915-1

Table 6.

Effects of intratesticular injections on plasma membrane functionality (PMF), DNA damage, viability and abnormal morphology of donkey testicles in different experimental groups. Values are expressed as mean ± SEM

Groups		Control	NS	HM	HS
Analysis		Control	NS	HM	HS
Sperm plasma membrane functionality (%)		90.68 ± 3.22^a	86.65 ± 1.93^b	39.87 ± 1.26^d	46.19 ± 1.74^c
DNA damage (%)		1.73 ± 0.20^b	2.08 ± 0.17^b	18.93 ± 0.90^a	17.75 ± 0.87^a
Viability (%)		89.19 ± 2.60^a	86.68 ± 3.32^a	37.19 ± 1.61^c	41.22 ± 1.19^b
Abnormalities sperm morphology %	Head abnormality %	8.22 ± 1.19^b	7.47 ± 0.78^b	29.36 ± 1.19^a	31.80 ± 1.45^a
	Tail abnormality %	4.15 ± 0.58^c	3.80 ± 0.41^c	16.62 ± 1.43^b	20.38 ± 0.61^a
	Proximal droplet %	12.31 ± 1.24^b	10.19 ± 1.21^b	23.08 ± 1.21^a	21.35 ± 1.77^a
	Distal droplet %	11.79 ± 1.44^b	9.73 ± 1.86^b	31.82 ± 1.35^a	28.79 ± 1.30^a
	Normal %	68.42 ± 2.71^b	65.80 ± 1.08^b	21.63 ± 0.71^a	20.77 ± 0.56^a

NS normal saline, HM hypertonic mannitol, HS hypertonic saline

^a,b,c,dDifferent superscripts within the same row demonstrate significant differences (p ≤ 0.05)