. 2024 Mar 11;20:99. doi: 10.1186/s12917-024-03915-1

Table 7.

Influence of intratesticular injections on histological parameters of donkey testicles in different experimental groups. Values are expressed as mean ± SEM

Groups	Control	NS	HM	HS
Analysis	Control	NS	HM	HS
Scrotal circumference (cm)	24.30 ± 1.45^a	24.00 ± 1.62^a	16.18 ± 1.54^b	15.60 ± 1.26^b
Testicular length (cm)	8.45 ± 0.90^a	8.09 ± 0.85^a	5.25 ± 0.93^b	4.78 ± 0.89^b
Testicular width (cm)	6.41 ± 0.48^a	6.35 ± 0.41^a	4.10 ± 0.95^c	5.19 ± 0.81^b
Testicular height (cm)	6.73 ± 0.71^a	6.90 ± 0.37^a	4.46 ± 0.74^c	5.54 ± 0.96^b
Testicular volume (cm³)	224.41 ± 25.64^a	220.52 ± 31.19^a	59.21 ± 8.13^c	68.12 ± 11.40^b
TBS (Johnsen’s score)	9.13 ± 0.24^a	8.70 ± 0.46^a	3.85 ± 0.21^b	3.42 ± 0.44^b
STD (µm)	269.41 ± 24.30^a	248.52 ± 39.95^a	65.96 ± 17.43^c	71.45 ± 19.78^b
Sertoli cell index (SCI)	24.75 ± 1.25^a	24.83 ± 1.20^a	4.21 ± 0.58^b	4.78 ± 0.34^b
Repopulation index (RI)	91.47 ± 3.18^a	88.64 ± 3.51^a	17.85 ± 1.62^b	16.09 ± 0.67^b
Miotic index (MI)	2.43 ± 0.31^a	2.51 ± 0.19^a	0.84 ± 0.25^b	0.71 ± 0.49^b
LCND (µm)	7.28 ± 0.32^a	6.81 ± 0.45^a	2.79 ± 0.17^b	3.15 ± 0.22^b
SCND (µm)	8.74 ± 0.27^a	8.30 ± 0.67^a	5.26 ± 0.73^c	4.79 ± 0.31^b
TDI (%)	92.43 ± 3.91^a	92.07 ± 2.84^a	16.23 ± 0.84^b	18.11 ± 1.08^b
SPI (%)	94.82 ± 2.48^a	92.84 ± 2.62^a	40.56 ± 1.55^c	45.70 ± 1.42^b

NS normal saline, HM hypertonic mannitol, HS hypertonic saline, TBS Testicular biopsy score, STD Seminiferous tubule diameter, LCND Leydig nuclear diameter, SCND Sertoli nuclear diameter, TDI Tubule Differentiation Index, SPI Spermiogenesis index

^a,b,cDifferent superscripts within the same row demonstrate significant differences (p ≤ 0.05)