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. 2024 Feb 20;10(2):001206. doi: 10.1099/mgen.0.001206

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© 2024 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.

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Fig. 1. — Genome-scale metabolic model (GSMM) overview. (a) Summary of the typical approach for generating a GSMM. (b) Constituent components of the Biomass Objective Function (BOF), which defines the requirements for production of new daughter cells. Optimization of the BOF via constraint-based analysis (c) can be used to predict growth in different conditions with an optimized objective value ≥0.001 indicating growth whereas an objective value <0.001 indicates no growth. (c) Common applications of GSMMs.