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. 2024 Feb 15;10(2):001199. doi: 10.1099/mgen.0.001199

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© 2024 to this article is held by Guangzhou KingCreate Biotechnologies Co.

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Fig. 1. — Validation of quantitative mNGS by KCQ assay with that of ddPCR. Total nucleic acid was extracted from samples and was added to reactions along with IS. The library was then prepared and sequenced by NGS. Bioinformatics analysis is able to obtain the IS sequences captured during the sequencing process. An IS regression-curve is prepared for the quantitation of microbial copy numbers before a quantitative mNGS test report is generated. Parallely, the same NA was used in ddPCR assay and the resulting copy numbers were compared to those obtained by KCQ.