Figure 2.

Pou4f1 binds to key regulators for RGC development. (A) Venn diagram depicting the overlap of genes associated with Pou4f1-bound elements displayed in Figure 1B and RGC-enriched genes from our E16.5 scRNA-seq dataset. (B) Quantitative ChIP-PCR validation of a selective subset of Pou4f1-bound peaks. (C) Diagram illustrating the known genetic regulatory network (GRN) in RGC development. Genes are categorized according to their involvement in development from retinal progenitor cells (RPCs) to matured RGCs. Genes harboring Pou4f1-enriched peaks near their gene bodies are highlighted in colored boxes and indicated with bold letter. The known GRN hierarchic edges are indicated with black or gray arrow and black dotted lines, and the novel GRN hierarchic edges are indicated with red lines.