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Purification of hairpin-ended and nicked hairpin-ended DNA substrates. Lane 1, ligation products of oligonucleotides used to construct the hairpin-ended DNA. Lane 2, hairpin-ended DNA after gel purification. Lane 3, ligation products of oligonucleotides used to construct the nicked hairpin-ended DNA. Lane 4, nicked hairpin-ended DNA after gel purification. The DNA samples were resolved by denaturing polyacrylamide gel electrophoresis.
