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Mutations that increase the stability of the U5-IR stem rescued the transposition defect of the 6-nt loop. Shown here are the sequences and the transposition activities of strains that contained modifications in the 6-nt loop version of the U5-IR stem-loop. The base pair in the stem adjacent to the loop was changed from AU to GC in the versions of Tf1 designated AG loop, CG stem, and RSV loop. WT, strain with a wild-type version of Tf1.
