TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide | Sequence (5′ to 3′)a | Mutation (strandb) |
|---|---|---|
| General | ||
| HL38 | GGAAGAGGAATCCTGGC | All (T) |
| HL39 | CCAATTGCTTCCAGTCTTTG | All (B) |
| Single-point mutation | ||
| HL260 | CCAAACTGCCTAGCTAACAATAACTG | G162C (T) |
| HL261 | CAGTTATTGTTAGCTAGGCAGTTTGG | G162C (B) |
| HL262 | CCTTAAATCAAAAACCAAACTGCG | U151A (T) |
| HL263 | CGCAGTTTGGTTTTTGATTTAAGG | U151A (B) |
| HL264 | CAAATACCAAACTCCGTAGCTAAC | G160C (T) |
| HL265 | GTTAGCTACGGAGTTTGGTATTTG | G160C (B) |
| HL266 | GGAAAACTCACCGGAGTTCTACGTATCC | C126G (T) |
| HL267 | GGATACGTAGAACTCCGGTGAGTTTTCC | C126G (B) |
| HL268 | GGAAAACTCACGGCAGTTCTACG | C124G (T) |
| HL269 | CGTAGAACTGCCGTGAGTTTTCC | C124G (B) |
| HL303 | GCAGTTCTACGTTTCCTTAAATC | A137U (T) |
| HL304 | GATTTAAGGAAACGTAGAACTGC | A137U (B) |
| HL380 | AAATCAAATAGCAAACTGCGTAGC | C153G (T) |
| HL381 | CGCAGTTTGCTATTTGATTTAAGG | C153G (B) |
| HL382 | GTTCTACCTATCCTTAAATCAAATACC | G135C (T) |
| HL383 | AAGGATAGGTAGAACTGCGGTGAG | G135C (B) |
| HL384 | TACCAAAGTGCGTAGCTAACAATAACTG | C158G (T) |
| HL385 | GCTACGCACTTTGGTATTTGATTTAAGG | C158G (B) |
| HL386 | CACCGCACTTCTACGTATCCTTAAATC | G128C (T) |
| HL387 | GTAGAAGTGCGGTGAGTTTTCCTTGCG | G128C (B) |
| Multiple-point mutation | ||
| HL305 | CTACGTATGGAAAAATCAAATACC | MSL2 (T) |
| HL306 | TTTGATTTTTCCATACGTAGAACTGCGG | MSL2 (B) |
| HL307 | CTACGTATTTCCAAATCAAATACC | MSL3 (T) |
| HL308 | TTTGATTTGGAAATACGTAGAACTGCGG | MSL3 (B) |
| HL309 | CGTATCCTTTTTTCAAATACCAAACTGCG | MSL4 (T) |
| HL310 | GGTATTTGAAAAAAGGATACGTAGAACTGC | MSL4 (B) |
| HL311 | CGTATCCTTGGGTCAAATACCAAACTGCG | MSL5 (T) |
| HL312 | GGTATTTGACCCAAGGATACGTAGAACTGC | MSL5 (B) |
| HL313 | CGTATCCTTAAAAGTTATACCAAACTGCG | MSL6 (T) |
| HL314 | CGCAGTTTGGTATAACTTTTAAGGATACG | MSL6 (B) |
| HL315 | CGTATCCTTAAACTGGATACCAAACTGCG | MSL7 (T) |
| HL316 | CGCAGTTTGGTATCCAGTTTAAGGATACG | MSL7 (B) |
| HL376 | AGTTGATGGTATCCTTAAATCAAATACC | MSL1 (T) |
| HL377 | GATACCATCAACTGCGGTGAGTTTTCC | MSL1 (B) |
| HL378 | TCAAATACGTAACTGCGTAGCTAAC | MSL8 (T) |
| HL379 | CGCAGTTACGTATTTGATTTAAGGATACG | MSL8 (B) |
| Deletion mutation | ||
| HL253 | GGAAAACTCACΔTAGCTAACAATAACTG | Deletion of nt 124 to 162 (T) |
| HL254 | TTATTGTTAGCTAΔGTGAGTTTTCCTTGCG | Deletion of nt 124 to 162 (B) |
| HL256 | GCTACGCAGTTTGΔGTAGAACTGCGG | Deletion of nt 135 to 153 (B) |
| HL270 | AGTTCTACΔCAAACTGCGTAGCTAAC | Deletion of nt 135 to 153 (T) |
| HL374 | TTCTACGTATΔATACCAAACTGCG | Deletion of nt 139 to 149 (T) |
| HL375 | TTTGGTATΔATACGTAGAACTGCGG | Deletion of nt 139 to 149 (B) |
| HL430 | AGTCCTACΔCAGACTGCGTAGCTAAC | GC-stem (T) |
| HL431 | AGTCTGΔGTAGGACTGCGGTGAGTTTTCC | GC-stem (B) |
| HL432 | AGTCATACΔCGGACTGCGTAGCTAAC | AG-loop (T) |
| HL433 | AGTCCGΔGTATGACTGCGGTGAGTTTTCC | AG-loop (B) |
| HL434 | AGTCAGAAGGACTGCGTAGCTAAC | RSV-loop (T) |
| HL435 | AGTCCTTCTGACTGCGGTGAGTTTTCC | RSV-loop (B) |
a
Mutated nucleotides are shown in boldface type. Δ, deleted nucleotides.
b
T, top; B, bottom.