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. 2024 Mar 7;19:2341–2357. doi: 10.2147/IJN.S449160

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© 2024 Zhao et al.

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Biocompatibility of rGOFs. (A) The RSC96 cells were co-cultured with rGOF extracts of different concentrations for 24 h to obtain the results of the calcein/PI cell viability/cytotoxicity assay kit (green: live cells, red: dead cells). Scale bar: 100 μm. (B) The results of live cell count obtained by the calcein/PI cell viability/cytotoxicity assay kit and their statistical analysis. The date is shown as the mean ± SD (n = 6) (C) The number of live cells was counted and statistically analyzed when the RSC96 cells were co-cultured with rGOFs extracts of different concentrations for different time length by using cell counting kit-8. The date is shown as the mean ± SD (n = 6) (D) H&E staining of various organs of rats with different types of defective neural connections at 12 weeks after surgery. Scale bar: 50 μm. n = 3; ns: no statistical difference among the groups.