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. Author manuscript; available in PMC: 2024 Mar 11.
Published in final edited form as: Appl Magn Reson. 2023 Oct 9;55(1-3):317–333. doi: 10.1007/s00723-023-01624-w

Figure 2.

Figure 2.

Stack plots of vertically offset 100 G X-band EPR spectra for control-subtracted HeLa cells treated with Ac4ManNAz (1), Ac4GlcNAz (2) or 9-Az-Sialic acid (3) that were (A) fixed with PFA and then reacted with DBCO-SL 4 or (B) reacted with DBCO-SL 4 and then fixed with PFA (dotted lines). Spectra are plotted with normalized integral areas to reflect mobilities. Annotations for the h1 and h0 transition area shown. Values for h1/ho given in the figure are evaluated for spectra from conditions where the cells were fixed and then spin-labeled. (C) Relative results of spectral components from fitting with Easy Spin where τR ≤ 2 ns is designated as “fast”, 2 ns < τR ≤ 10 ns is designated as “intermediate”, and τR > 10 ns is designated as “slow”. Numbers reflect the relative percentages.