FIG. 3.

gas5 is member of the 5′TOP gene family. (a) Extension of a deoxyoligonucleotide primer complementary to mouse gas5 (lane 5) was performed using mRNA isolated from NIH 3T3 cells treated with cycloheximide. The products were run adjacent to a sequencing ladder of DNA containing the mouse gas5 exon 1 and promoter (lanes 1 to 4). (b) The transcription start site maps to two adjacent cytidine residues located 29 and 30 nucleotides downstream of a TATA box and 32 and 33 nucleotides upstream of a consensus translation start site. The TATA element, transcription start sites, and translation start site are boxed; the position of the first intron of the mouse gas5 gene is indicated.