FIGURE 5.
Predicted secretion profile implications in chronic wound healing. Wound-healing scoring based off of secretion profile measured from the multiplex immunoassay, compared to data from healing chronic wound fluid in Ligi et al.24 The authors also used the same BIO-RAD Multiplex assay to perform measurements of the same 27 inflammatory factors. Significant pro-wound-healing trends for nine factors were identified as human venous ulcers began healing. The resulting secretion profiles/fold changes from Hp, Hb, or Hb-Hp in the current study were compared to media baseline to determine the experimental trend. The treatment trends were then compared to the pro-wound-healing trends from Ligi et al. If the experimental trend was consistent with the pro-wound-healing trend and had an absolute value fold change of 0.1 to 1, +1 point was awarded. If the trend was consistent and had an absolute value fold change of >1, +2 points were awarded. If the trends were inconsistent, points were subtracted rather than added. If the fold change value was between >−0.1 and <0.1, no points were awarded. The table shows the score for each treatment for each pro-wound-healing trend, and the number line shows the total score for each treatment