Fig. 4.

Member of the Dma1 regulon, NigD1 (BT_4005), is necessary to induce vesiculation. Volcano plot representations of (A) transcriptome and (B) proteome data comparing WT and Δdma1. (C) Coomassie blue stain of TM and OMV fractions isolated from 1. WT, 2. Δdma1, 3. Δ1287/dma1, 4. ΔnigD1/dma1, and 5. ΔnigD2/dma1. Left: The protein profile of mutant TMs is the same as WT, indicating that all changes are specific to the OMV fraction. Right: Deletion of NigD1 in the Δdma1 background restores the protein profile to WT. Samples were normalized by OD600 and run on 10% SDS-PAGE.