Extended Data Fig. 8. Comparison of FBP3 pathway to luciferases that require exogenous substrate in HEK293NT cells.

(a) Integral luminescence signal collected over 20 minutes. The boxes are the first and the third quartiles, whiskers are the rest of the distribution except outliers, the orange line is the median. N = 5 biologically independent samples per box plot. The difference between mean values and p-values of post-hoc two-sided Mann-Whitney U-test corrected by the step-down method using Sidak adjustments are indicated below the brackets between the box plots. (b) Kinetics. Data shown as mean (solid line) ± SD (area around the solid line). For NanoLuc, 0.5 µL of substrate N113 (N1110, Promega) was used. For FFLuc, we used 100 µM of D-Ln. For nnLuz v4, 100 µM of fungal luciferin was added. For FBP3, 100 µM of caffeic acid.