Extended Data Fig. 7. Comparison of FBP3 pathway to luciferases that require exogenous substrate in BY-2 cells.

(a) Integral luminescence signal collected for 30 minutes. The boxes are the first and the third quartiles, whiskers are the rest of the distribution except outliers, the orange line is the median. N = 5 plant cell packs for NanoLuc and FBP3, and N = 8 for FFLuc. The difference between mean values and p-values of post-hoc two-sided Mann-Whitney U-test corrected by the step-down method using Sidak adjustments are indicated below the brackets between the box plots. (b) - Kinetics. Data shown as mean (solid line) ± SD (area around the solid line). For NanoLuc, 0.75 µL of substrate N113 (N1110, Promega) was used. For FFLuc, we used 100 µM of D-Ln. For FBP3, no substrate was added.