FIG. 6.

The atypical E-box elements in the c-Kit promoter do not bind Mi. (A) Oligonucleotides used as probe and competitors in the DNA binding assay. (B) Band shift assay using an M-box probe and bacterially expressed and purified Mi together with the indicated competitors at 10, 50, and 250 ng. (C) Yeast one-hybrid assay for binding of VP16 Mi to the M box and c-Kit E-box elements. (D) Yeast one-hybrid assay for binding of MyoD to the M box and c-Kit E-box elements. Yeast were transformed with the indicated CYC-lacZ reporters together with vectors expressing either an Mi-VP16 fusion protein or MyoD were assayed for β-galactosidase activity. The sequences of the oligonucleotides cloned into CYC-lacZ reporter plasmids are listed in Table 1.