TABLE 1.
Sequences of oligonucleotides used for DNA binding assays and as targets in the yeast CYC-lacZ reportersa
| Method description | Oligonucleotide sequence |
|---|---|
| DNA binding assays | |
| mTRP-1 M box | 5′-ctagaCAGTGGGGAGGGGAGTCATGTGCTGCCTAGTATt |
| mTRP-1 M box T→A | 5′-ctagaCAGTGGGGAGGGGAGaCATGTGCTGCCTAGTATt |
| mTRP-1 M box T→C | 5′-ctagaCAGTGGGGAGGGGAGcCATGTGCTGCCTAGTATt |
| mTRP-1 M box T→G | 5′-ctagaCAGTGGGGAGGGGAGgCATGTGCTGCCTAGTATt |
| CAC M box | 5′-ctagaCAGTGGGGAGGGGAGTCAcGTGCTGCCTAGTATt |
| hTyros InrE | 5′-gatctCTCAGAGCCAAGACATGTGATAATCACTGTAGTAGTa |
| hTyros InrE m1 | 5′-gatctCTCAGAGCCAAGACATGTGcTAATCACTGTAGTAGTa |
| mTyros InrE | 5′-ctagaCTTAGCCAAAACATGTGATAGTCACTCCAGGt |
| hTyros TDE | 5′-ctagaCTTGTGGAGATCATGTGATGACTTCCTGATTCCt |
| hTyros TDE m1 | 5′-ctagaCTTGTGGAGAaCATGTGATGACTTCCTGATTCCt |
| hTyros TDE m2 | 5′-ctagaCTTGTGGAGATCATGTGcTGACTTCCTGATTCCt |
| hTyros TDE m3 | 5′-ctagaCTTGTGGAGAaCATGTGcTGACTTCCTGATTCCt |
| c-Kit (E) | 5′-ctagaAGGGAGCACCTGCCAGGTGGCTGGCt |
| MB1 | 5′-ctagaCTCGAGGGGCCTGCCATGTGCACCCCGCCCGGGt |
| MB2 | 5′-ctagaCTCGAGGCACAAGACATGTGCTTAGCAAACGGGt |
| MB2 A→T | 5′-ctagaCTCGAGGCACAAGtCATGTGCTTAGCAAACGGGt |
| MB3 | 5′-ctagaCTCGAGACTACAGACACGTGCCACCACACCCAAt |
| P gene | 5′-ctagaAGCTGTGGGCGGGGCCATGTGGGCAGCTTTGTTt |
| Yeast expression | |
| M box | 5′-agatctctcgagCAGTGGGGAGGGAGTCATGTGCTGCCTAGTATCCAGTGGGGAGGGAGTCATGTGCTGCCTAGTATggatcc |
| c-Kit | 5′-agatctctcgagAGGGAGCACCTGCCAGGTGGCTGGCATAGGGAGCACCTGCCAGGTGGCTGGCggatcc |
| MB2 | 5′-agatctctcgagGCACAAGACATGTGCTTAGCAAACGGGATCGCACAAGACATGTGCTTAGCAAACGGGATggatcc |
| MB2 A→T | 5′-agatctctcgagGCACAAGtCATGTGCTTAGCAAACGGGATCGCACAAGtCATGTGCTTAGCAAACGGGATggatcc |
a
Only one strand is shown. The E-box elements are underlined, and additional bases used to create restriction enzyme sites at the termini are shown in lowercase letters. Lowercase is also used to indicate the positions of specific mutations referred to in the text. Arrows indicate residue substitutions.