Table 1.

Synthetic chimeric enzymes with improved physicochemical properties over native enzymes

Enzyme	EC number	Organism	Domain Added	Domain origin	Improved Property	Refs.
Laccase	EC 1.10.3.2	Pleurotus ostreatus	Complete class I hydrophobin Vmh2	Pleurotus ostreatus	• Increased immobilization yield	[25]
Glicosylhidroolase	EC 3.2.1	Thermotoga maritima	Dockerin domain	Piromyces finnis	• Thermostability	[26]
Glucoamylase GATE	EC 3.2.1.3	Talaromyces emersonii Ld418 TE	Starch-Binding Domain of glucoamylase GAA1	Apergillus niger Ld418A1	• Enzyme activity • pH stability	[27]
Xylanase	EC 3.2.1.8	Thermobacillus xylanilyticus	N-terminal of GH11 xylanase	Neocallimastix patriciarum	• Wider substrate specificity	[28]
Xylanase A AnxA	EC 3.2.1.8	Aspergillus niger	N-terminal of xylanase A TfxA	Thermomonospora fusca	• Thermostability • Catalytic activity	[23]
β-xylanase	EC 3.2.1.8	B. subtilis	A complete β-xylosidase	B. subtilis	• Substrate cleavage rate • Optimum temperature • Thermostability	[29]
Cyanide dehydratase	EC 4.2.1.66	B. pumilus	C-terminal of cyanide dehydratase	P. stutzeri	• Thermostability • Optimum pH • Operational pH range	[30]
Nitrile hydratase	EC 4.2.1.84	P. putida NRRL-18,668	N-terminal of nitrile hydratase C-terminal of nitrile hydratase	Comamonas testeroni 5-MGAM-4D P. thermofila JCM3095	• Enhancement in thermostability • More tolerant to high-concentration product • Increase in activity	[31]

CMB Carbohydrate binding module GH Glycosyl hydrolase