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. 2024 Mar 12;8:43. doi: 10.1186/s41747-024-00448-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Grayscale images of two whole hematoxylin-eosin -stained slides and corresponding histograms of pixel distribution after treatment. a Grayscale image of a tumor in the bufalin plus sorafenib treatment group (histopathological standard deviation 10.475). b Grayscale image of a tumor in the control group (histopathological standard deviation 13.795). c Histograms of pixel distributions in the grayscale images of the tumors in the bufalin plus sorafenib treatment (blue) and control groups (orange)