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. 2024 Jan 24;32(3):689–703. doi: 10.1016/j.ymthe.2024.01.025

Figure 6.

Figure 6

Aerosolization of COVA2-15 and 2E8 SIgA1 antibodies using the Omron MicroAir U22 portable mesh nebulizer; mAb, 1 mL, with a concentration of 100 μg/mL was nebulized and condensate was collected

(A) Protein concentration in the condensate after aerosolization was measured using absorbance at 280 nm. (B) Antigen binding of samples before and after nebulization was tested by ELISA with equal protein loading. Values for recovery and antigen-binding activity are means ± SDs of at least 3 independent aerosolization experiments. (C) Overlay of representative size-exclusion chromatograms of mAbs before and after nebulization. Curves are representatives out of 3 repeats with similar results. A total of 200 μg of SIgA1 were injected onto a Superdex 200 Increase 10/300 GL column.