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. 2024 Mar 11;15(3):203. doi: 10.1038/s41419-024-06568-y

Fig. 2. CRISPR‒Cas9 screening identified key genes involved in protein integration into the ER membrane.

Fig. 2

a A scheme showing the key steps used to conduct a pooled genome-wide CRISPR‒Cas9 screen. b Enrichment of gRNAs in the populations of cells sorted for either increased or decreased mVenus fluorescence. The gene rank and enrichment score were obtained from the robust rank aggregation method from 6 biological replicates (3× Clone 1 and 3× Clone 2). c A scheme showing the process by which the genes were selected for arrayed screening. d, e Target validation of 29 significantly enriched gRNAs from either the high fluorescence (d) or the low fluorescence (e) populations, showing that 25 (red) and 13 (blue) guide RNAs significantly (P ≤ 0.05) alter mVenus fluorescence. Mean ± S.D, n = 4 (2× Clone 1 and 2× Clone 2) mixed effects models with significance tests performed using Satterthwaite’s degrees of freedom method with ImerTest.