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. 2024 Mar 11;15(3):203. doi: 10.1038/s41419-024-06568-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b GET4 and BAG6 interact with IP3R and GRP75, respectively. ER-Mito reporter cell lysates prepared from either clone 1 (a, b) or clone 2 (c, d) were incubated with Dynabeads^TM and either anti-GET4 or anti-BAG6 antibodies. Cell lysates were analysed with the indicated antibodies. e IP-mass spectrometry samples for GET4 and BAG6 detection. ER-Mito reporter cell lysates were incubated with Dynabeads^TM and either anti-GET4 or anti-BAG6 antibodies. These were analysed by western blotting, probed with the indicated antibodies and sent for mass spectrometry analysis.