FIG. 11.

(A) Growth characteristics of the yeast strains carrying PKR point mutants. Growth of transformed yeast strains containing pYES2 alone (diamonds), wt PKR/pYES2 (closed squares), K296R/pYES2 (closed triangles), K60A/pYES2 (open triangles), A63/pYES2 (open circles), K60A,A63E/pYES2 (open squares), and A67E/pYES2 (closed circles) was assayed in synthetic medium lacking uracil and containing 2% galactose. (B) Western blot analysis. Yeast cell extracts were examined by Western blot analysis using a MAb against PKR; 100 μg of total protein was loaded in each lane. Lane 1, pYES2 vector alone; lane 2, K296R/pYES2; lane 3, K60A/pYES2; lane 4, wt PKR/pYES2; lane 5, A63E/pYES2; lane 6, K60A,A63E/pYES2; lane 7, A67E/pYES2. The arrow represents the position of the PKR protein.