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. Author manuscript; available in PMC: 2024 Mar 12.
Published in final edited form as: Nat Med. 2023 Feb 23;29(3):700–709. doi: 10.1038/s41591-023-02217-7

Extended Figure 6. Heterologous expression of thiolase and acyl-CoA N-acyltransferase enzymes in E. coli BL21.

Extended Figure 6.

(A) Coomassie-stain gel shows purity of indicated enzymes after overexpression and cobalt purification. N=1 protein purification per enzyme; repeated independently n=2-4 for each enzyme. Abbreviations: FcTHL = a predicted thiolase (R6CZ24) from an uncultured Firmicutes; FpGNAT = a predicted acyl CoA N-acyltransferase (C7H1G6) from F. prausnitzii; StNAT = a known arylamine N-acetyltransferase from Salmonella enterica serovar typhimurium LT2. (B) Confirmation of in vitro acetylation of 5-ASA by known S. typhimurium enzyme using 1 mM of each substrate and 5 uM enzyme incubated for 1hr at RT. Data are presented as mean values +/− SEM.