Table 2. Generation of the resting state model using a combination of multiple templates for different voltage-sensing domains (VSDs) and pore domains (PDs) of Naᵥ1.7.

Template pdb	Domains used	Res IDs (from template)	Res IDs (human Naᵥ1.7 numbering)	Template info
7xve	VSDI, PDI, PDII	1–404, 541–650	8–411, 864–972	Naᵥ1.7 mutant (L866F, T870M, and A874F on S5II; V947F, M952F, and V953F on S6II; and V1438I, V1439F, and G1454C on S6III, E156K on S2I and G779R on S2II) VSDI deactivated (VSDII partially deactivated but not used)
7k48	VSDII, PDII, VSDIII, PDIII, PDIV	405, 938, 1115–1244	728–973, 1175–1462, 1639–1768	Naᵥ1.7/NaᵥAb chimera, where top half of each VSD is Naᵥ1.7 VSDII; all VSDs deactivated with engineered tarantula toxin m3-Huwentoxin-IV bound
6nt4	VSDIV, PDIV, PDI	939–1244, 239–404	1463–1768, 246–411	Naᵥ1.7/NaᵥPas chimera, full Naᵥ1.7 VSDI, and VSDIV deactivated with α-scorpion neurotoxin AaH2 bound
7w9k	Loops between S5 and S6 regions for PDII, PDIII, and PDIV	563–619, 811–918, 1141–1213	886–942, 1335–1442, 1665–1737	Fast-inactivated Naᵥ1.7 (all VSDs activated) DIII–IV linker bound to pore