Fig. 3.

Depletion of LRP6 suppresses TNBC cell proliferation/viability. (A, B) MDA-MB-231 and BT549 cells in 96-well plates were transiently transfected with 50 nM of LRP6 siRNA-1, LRP6 siRNA-2 or control siRNA. After 24, 48, 72 or 96 h of transfection, cell viability was measured by the Cell Titer Glo Assay system. Data shown are representative of three independent experiments. All the values are the average of triplicate determinations with the s.d. indicated by error bars. *P < 0.05, **P < 0.01 versus control cells. (C, D) MDA-MB-231 and BT549 cells were transiently transfected with 50 nM of LRP6 siRNA-1, LRP6 siRNA-2 or control siRNA. After 96 h incubation, images were taken using phase contrast microscopy. Scale bar: 40 μm.