Fig. 6.

LRP6 depletion and Mesd treatment inhibit S100A4 expression in TNBC cells. (A) MDA-MB-231 and BT549 cells in 6-well plates were transiently transfected with 50 nM of LRP6 siRNA-1, LRP6 siRNA-2 or control siRNA. After incubation of 48 h, the levels of S100A4 expression were examined by Western blotting. (B) MDA-MB-231 and BT549 cells in 6-well plates were treated with Mesd at the indicated concentrations for 24 h. The levels of S100A4 were examined by Western blotting. Data shown are representative of three independent experiments. All the samples were also probed with anti-human actin antibody to verify equal loading.