FIGURE 1.

Fisetin suppresses cellular senescence in endothelial cells. Cell viability in replicating (control) and senescent human umbilical vein endothelial cells (HUVECs) to increasing doses of fisetin (dashed line represents the IC₅₀). IC₅₀ values of control and senescent HUVECs with 95% confidence intervals. Viability of control and senescent HUVECs at 1 μM fisetin (n = 4–8) (a). Senescence‐associate beta galactosidase (SA‐β‐Gal) signal in control and senescent HUVECs to increasing doses of fisetin (n = 3) and representative images, right (b). mRNA gene expression of cellular senescence markers Cdkn2a and Cdkn1a in control, senescent, or 1 μM fisetin‐treated senescent human aortic endothelial cells (HAECs) (n = 3) (c). Values represent mean ± SEM. *p < 0.05 senescent versus senescent + treatment; ^p < 0.05 versus control.