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. 2024 Feb 20;13(2):644–650. doi: 10.21037/tcr-23-1938

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2024 Translational Cancer Research. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Immunohistochemical images and AOD values of tissue microarrays after time gradient decalcification or depigmentation. (A) Positive immunohistochemical staining for PD-L1 at different decalcification times 6, 12, 24, 36, and 48 hours; (B) positive immunohistochemical staining for PD-L1 at different depigmentation times 1, 5, 15, 30, and 60 min; (C) AOD value of average optical density at different decalcification times of 6, 12, 24, 36, and 48 hours; (D) different depigmentation time 1, 5, 15, 30, 60 min average optical density AOD value. *, P<0.05; **, P<0.01. AOD, average optical density; PD-L1, programmed death ligand 1.