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. 2024 Feb 20;13(2):644–650. doi: 10.21037/tcr-23-1938

Figure 2.

Figure 2

Various PD-L1 scores and positive rates of esophageal cancer samples after decalcification. PD-L1 (22C3) staining was performed on the Dako Autostainer Link 48 platform (A) PD-L1 expression in 10 PD-L1 positive esophageal squamous cell carcinoma samples before and after 24 and 36 h decalcification treatment; (B) after 24 h decalcification, the CPS, TPS and IPS of PD-L1 decreased, and the PD-L1 positivity rate was 90%; (C) after 36 h decalcification, the CPS, TPS, and IPS scores of PD-L1 decreased, and IPS and TPS significantly decreased, and PD-L1-positive rate was 50%. *, P<0.05; **, P<0.01. CPS, Combined Positive Score; TPS, Tumor Proportion Score; IPS, Immunocyte Proportion Score; PD-L1, programmed death ligand 1.