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Percentage of total p-Tyr contained in individual p190 phosphopeptides from 32P-labeled IV5 cells
| Peptide no. | p-Tyr contenta
|
|
|---|---|---|
| PIU | % of total p-Tyr in p190 | |
| 3 | 27,100 | 79.8 |
| 8 | 5,072 | 14.9 |
| 24 | 595 | 1.8 |
| 25 | 1,087 | 3.2 |
| 27 | 115 | 0.3 |
| Total | 33,969 | 100 |
Quantitated by PhosphorImager analysis and expressed in PIU. The sum of PIU from all p-Tyr-containing peptides was taken as the value for total p-Tyr in p190. The percentage of the total p-Tyr contained in each peptide was then determined.
