Extended Data Fig. 8. Altered signaling in activated SCAP deficient B cells.

B cells isolated from SCAP^+/+CD19^Cre/+ mice and SCAP^fl/flCD19^Cre/+ mice were stimulated with 10 μg/ml LPS, 5 μg/ml anti-CD40 or 20 μg/ml anti-IgM for 0, 4 and 24 hours. Cell lysates were analyzed by immunoblotting. a. Representative immunoblotting. b. Quantitation of 2 independent immunoblotting experiments. c. B cells isolated from SCAP^+/+ CD19^Cre/+ mice and SCAP^fl/fl CD19^Cre/+ mice were stimulated with 10 μg/ml LPS and cultured with or without 5 μg/mL MβCD conjugated cholesterol for 24 hours. Data shown is a representative immunoblotting of 2 independent experiments.