Extended Data Fig. 10. SREBP signaling regulates GC B cell response.

a-b. AID-Cre R26^YFP mice were immunized with NP-OVA adjuvanted by RIBI through i.p or s.c. Splenocytes (i.p immunization) and dLN cells (s.c immunization) were analyzed by FACS 10 days post immunization. GC B cells and CD86^hi MHCII^hi non-GC B cells were compared for Cre activity through their YFP reporter expression. a shows the gating strategy; b shows the statistical analysis of 2 independent experiments (n = 5, mean ± SD). c. SCAP^+/+ AID-Cre R26^YFP mice (+/+) and SCAP^fl/fl AID-Cre R26^YFP mice (fl/fl) were i.p. immunized with NP-OVA adjuvanted with RIBI. Cells in the spleen were analyzed by FACS 14 days post immunization for the % of YFP⁺ GC B cells in total GC B cells (gated on CD19⁺CD95⁺CD38⁻ live singlets). 10 + /+ and 14 fl/fl mice from 4 independent experiments were analyzed. a-c, P values were determined by two-tailed unpaired t-test. ****P ≤ 0.0001. d. Splenocytes from day 14 RIBI adjuvanted NP-OVA immunization were analyzed by FACS to compared lipid raft content between GC and non-GC B cells. Lipid rafts were stained with cholera toxin subunit B (CT-B). Data show one representative of two independent experiments.