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. Author manuscript; available in PMC: 2024 Mar 12.
Published in final edited form as: Nat Immunol. 2022 Dec 28;24(2):337–348. doi: 10.1038/s41590-022-01376-y

Extended Data Fig. 1. SREBP signaling in CD11c+ antigen presenting cells is dispensable for modulation of antibody and CD8 T cell responses.

Extended Data Fig. 1

a. Spleen single cell suspension from SCAPfl/fl (n = 4) and SCAPfl/fl CD11c-Cre mice (n = 6) were analyzed by flow cytometry for dendritic cell (DC) subtypes. DC subtypes are gated as cDCs (CD45+CD11c+MHCII+) which include cDC1 (CD8a+CD11b−) and cDC2 (CD8a−CD11b+), pDCs (CD45+CD11cloPDCA1+). Graphs show the representative flow cytometry plots and the statistics for the frequencies of DC subsets. b. SCAPfl/fl and SCAPfl/fl CD11c-Cre mice were immunized subcutaneously with OVA adjuvanted with RIBI (n = 12 for SCAPfl/fl and n = 9 for SCAPfl/fl CD11c-Cre) or AddaVax (n = 8 for each group), and serum antibody titers at day 28 were measured by ELISA. c. SCAPfl/fl and SCAPfl/fl CD11c-Cre mice (n = 9 for each group) were immunized with 2×106 plaque-forming units (PFU) of YF-17D virus, and antigen specific CD8 T cells producing IFN-γ in the lung and liver were analyzed by flow cytometry. Graphs show frequencies of CD8 T cell (gated as CD3+CD8+CD4−) producing IFN-γ in the lung and liver with representative flow cytometry plots. a-c, Data represent two independent experiments, P values are determined by two-tailed unpaired t-test. ns: P > 0.05.