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Primers used in reverse transcription-quantitative PCR.
| No. | Gene name | Primer sequence (5'→3') |
|---|---|---|
| 1 | SOX9 | F: AAGCTCTGGAGACTTCTGAACG |
| R: CTGCCCGTTCTTCACCGACT | ||
| 2 | HSPA4 | F: GTGGACCTGCCAATCGAGAA |
| R: CCTCCACTGCGTTCTTAGCA | ||
| 3 | SMAD2 | F: TGGGGACTGAGTACACCAAA |
| R: GGGATACCTGGAGACGACCA | ||
| 4 | HIF1A | F: TTTGGCAGCAACGACACAGA |
| R: TTTCAGCGGTGGGTAATGGA | ||
| 5 | GSK3B | F: TGTGTGTTGGCTGAGCTGTT |
| R: TCCCTTGTTGGAGTTCCCAG |
F, forward; R, reverse.
