Figure 4.

Antibiotic administration downregulates the expression of tight junction proteins and suppresses gut microbial colonization. (A) The protein expression levels of TJP1, CLDN1 and OCLN in different groups. (B) The ratio of TJP1, CLDN1, or OCLN grayscale values to β-actin grayscale values in different groups (n=3). (C) Bacterial load in the colonic contents of different groups (n=5). ^*P<0.05 and ^**P<0.01, compared with the model group; ^{^}P<0.05 and ^{^^}P<0.01, compared with the control 2 group; ^#P<0.05 and ^##P<0.01, compared with the model 2 group; ^IP<0.05 and ^IIP<0.01. CLDN1, claudin-1; OCLN, Occludin; OVA, ovalbumin; ABX, antibiotics.