Table 3.

The list of buffers and reagents that were used for setting up SFNH, MS preparation, and harvesting of C. elegans.

Media/Buffer	Preparation
NGM agar	5 g NaCl, 17 g Bacto agar, 2.5 g Peptone, 975 mL 3D H2O after cooling to 55°C add: 1 mL 1 M CaCl, 1 mL 1 M MgSO4, 1 mL 5 mg/mL Cholesterol in ethanol, 25 mL 1 M KPO4 buffer
S-basal buffer	5.85 g NaCl, 1 g K2HPO4, 6 g KH2PO4, 1 L H2O
Bleach solution	1 mL stock bleach, 2.6 mL autoclaved 3D H2O, 0.4 mL NaOH
0.85% NaCl buffer	0.85 g NaCl and 1 L dH2O
M9 buffer	3 g KH2PO4, 6 g Na2HPO4, 5 g NaCl, 1 L H2O, and 1 mL 1 M MgSO4 added after autoclaving