Figure 8.

SMRT sequencing reveals transcriptional elongation defects following CDK12 knockdown during AKI. (A) Integrative Genomics Viewer (IGV) representation shows the mapping of SMRT sequencing reads at the Med16, Smug1, Txnip, Cast, and Fgf1 genes. The presence of elongation defect due to CDK12 knockdown in mice treated with cisplatin as in number of short transcrips. (B) Three primers (located near the 5′ untranslated regions, coding sequence, 3′ untranslated regions) are labeled in blue, pink and red, respectively. Expression of Med16, Smug1, Txnip, Cast, and Fgf1 at selected regions in different groups was assessed by real-time PCR. Error bars indicate mean values of ±SD, n=3.