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S. anginosus infection elicits significant upregulation of NF-κB and inflammatory mediators like NLRP3, iNOS and COX2. (A) Western blot analysis of RAW 264.7 cells treated with LPS, S. anginosus and S. mitis for NF-kB and NLRP3; (B). Densitometry analysis of western blot ; (C) mRNA expression of iNOS and Cox2 and (D) Protein expression of iNOS and Cox2 . The data are presented as Mean ± SEM. * P < 0.05 and ***P < 0.0001 compared to untreated RAW 264.7 cells (n = 3).
