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Timeline of immunization and sample collection. Female C57BL/6 mice (n = 5/group) received prime and boosts with PBS, VLPs, 5 or 10 µg Hcp1-VLPs formulation, via the intranasal route, at 2-week intervals. Baseline and post-immunization sera were collected a week prior to prime and 2 weeks after the second boost, respectively. This figure was created with Biorender (biorender.com).
