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. 1998 Dec;18(12):7139–7146. doi: 10.1128/mcb.18.12.7139

FIG. 4.

FIG. 4

Increased SAR1 dosage suppresses the invertase secretion defect in rse1-1 and prp2-13 strains. Wild-type (CKY294) (WT), sec12-4 (CKY39), and rse1-1 (CKY567) strains were incubated at 37°C for 2.5 h in YEP plus 0.1% glucose to induce invertase; the prp2-13 (CKY570) strain was shifted to 30°C for 2.5 h in YEP plus 0.1% glucose. Invertase was detected in protein extracts by SDS-PAGE and immunoblotting with anti-invertase antibody.