TABLE 2.

Splicing defects in rse1-1 and prp2-13 strains in a reporter assay

Host strain	% β-galactosidase activitya of:
	Construct with no intron	RP51 intron	Acc intron
Wild type	100	37.3 ± 2.9	10.6 ± 0.6
rse1-1	100	13.1 ± 2.8	1.7 ± 0.2
prp2-13	100	0 ± 0	0 ± 0

^aβ-Galactosidase activity is expressed as the percentage of activity from the intronless β-galactosidase construct under the same conditions. The means and standard deviations for at least two assays of each of three different transformants are given. The absolute values of β-galactosidase activity for the intronless β-galactosidase transformants in a typical experiment were as follows: wild-type, 640 units; rse1-1, 830 units; and prp2-13, 1,000 units. One β-galactosidase unit is defined as 1,000 × OD₄₂₀/min of reaction for 1 ml of culture at OD₆₀₀ of 1.