TABLE 3.
Restoration of invertase secretion in rse1-1 and prp2-13 strains by overexpression of SAR1a
| Strain | % Invertase secreted | Total invertase activity (units/OD600)b |
|---|---|---|
| Wild-type | 87 | 0.26 ± 0.03 |
| sec12-4 | 5 | 1.31 ± 0.07 |
| rse1-1 | 50 | 0.50 ± 0.06 |
| rse1-1 [SAR1 CEN] | 80 | 0.33 ± 0.02 |
| prp2-13 | 42 | 1.40 ± 0.15 |
| prp2-13 [SAR1 CEN] | 83 | 0.66 ± 0.04 |
a
To induce invertase, wild-type (CKY294), sec12-4 (CKY39), and rse1-1 (CKY567) strains were shifted to 37°C for 2 h in YEP plus 0.1% glucose and the prp2-13 (CKY570) strain was shifted to 30°C for 2 h in YEP plus 0.1% glucose.
b
The means and standard deviations from four assays (two each from two independent cultures) are given. One unit of invertase activity releases 1 μmol of glucose from sucrose at 30°C.