Figure 2.

Cell phenotypes and distribution in NSCLC

(A) Exemplary LUAD (left) and LUSC cores (right) after cell segmentation based on panCK as a tumor marker and SMA as a stromal marker. Top to bottom, cells colored by average expression of SMA and panCK; cell assignment based on the generated tumor-stroma masks; cell category assignment; and the binned distance of all non-tumor cells to the nearest tumor-stroma border (30 μm bins [red, orange, yellow, green, blue, purple] from tumor-stroma interface; gray: tumor).

(B) Heatmap of marker expression (normalized from 0 to 1) for all cell phenotypes (labeled cell types). The bar chart above the heatmap shows the number of cells (square root for visualization) of each cell phenotype per tumor type.

(C) Mean proportions of the indicated cell categories per patient, annotated for clinical features. Dist. Met.: distant metastasis; LN met.: lymph node metastasis; Neoad.: neoadjuvant therapy.

(D) tSNE of a subset of 1,000 cells per image colored by cell category.

(E) Proportions of cell categories for all cells measured. For D, E, colors as in B.