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. 2024 Mar 11;42(3):396–412.e5. doi: 10.1016/j.ccell.2023.12.021

Figure 6.

Figure 6

The spatial relationship of CAFs with the tumor microenvironment

(A) Heatmap showing enrichment or depletion of cell types between patient groups defined by CAF composition (1, 2, 3, and 4).

(B) Density curves showing the distance per cell of the indicated CAF types to the closest tumor-stroma border for LUAD (left) and LUSC (right).

(C) Neighborhood analysis heatmap showing nearest 15 neighbors of each cell within a radius of 20 μm (to cell, Y axis; from cell, X axis). Plotted are mean significances over all images, split by LUAD (left) and LUSC (right). Positive/negative (red/blue) mean scores indicate higher/lower cellular interactions compared to a random null-distribution.

(D) Images with mask overlays (white) of SMA CAFs (SMA+), mCAFs (MMP11+), collagen CAFs (Collagen I/Fibronectin+), vCAFs (CD146+), iCAFs (CD34+), tCAFs (CD10+), hypoxic CAFs (CAIX+). The general stroma marker SMA is in red in all cases except for images showing SMA CAFs and hypoxic CAFs. Pan cytokeratin is in magenta in all cases except for the image showing hypoxic CAFs (lower right), all CAFs are panCK−; DNA is in blue. Scalebars are in μm.