Quantification of proinflammatory cytokine load in the basal and vascular compartments of the airway chips following SARS-CoV-2 Italian isolate infection. The perfused media from the basal and the vascular sides of the units (infected [N = 3], control, [N = 3]) were collected at 24, 48, 72, 120, and 168 hours post infection. The amounts of 10 proinflammatory cytokines were analyzed in these samples by multiplexed ELISA, of which data for 10 cytokines (IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL12-p70, IL13, and TNF-α) are included. Each sample was included in the multiplexed ELISA as technical duplicates and the data were averaged for each analyte at each time point. The overall average of the biological replicates was obtained and represented as pg ml⁻¹ concentration for each cytokine. Statistical analysis was carried out using t-test *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

	Vascular					Airway
Time (HPI)	24	48	72	120	168	24	48	72	120	168
INF-γ	ns	*	*	ns	ns	***	**	**	ns	ns
IL-1β	***	*	*	*	ns	***	***	**	*	*
IL-2	ns	ns	ns	ns	ns	ns	ns	*	ns	ns
IL-4	ns	ns	*	ns	ns	***	***	**	*	**
IL-6	**	**	**	**	*	***	****	****	****	***
IL-8	***	***	***	***	**	****	****	****	****	ns
IL-10	ns	ns	ns	ns	ns	*	*	ns	ns	ns
IL-12p70	ns	ns	ns	ns	ns	***	**	**	*	**
IL-13	ns	ns	*	ns	ns	****	***	***	**	**
TNF-α	ns	ns	ns	ns	ns	*	*	ns	ns	ns