FIGURE 9.

Higher relative amiloride‐sensitive (ENaC) currents in cpk compared to wt cells. Whole‐cell currents were evoked by applying voltage steps from −90 to 30 mV in 20 mV increments. Cells were held at −30 mV. (A–C) Representative whole‐cell current recordings from wt cells; (D–F) Representative whole‐cell current recordings from cpk cells; (A and D) Representative whole‐cell current recordings in the absence of amiloride. (B and E) Representative whole‐cell current recordings in the presence of amiloride (10 μM). (G) Amiloride‐sensitive currents (C and F) were measured by subtracting (B) from (A) and (E) from (D). Mean I–V relationship of amiloride‐sensitive currents expressed by total currents for wt (open circle, n = 15) and cpk (dark circle, n = 13) cells. (H) Mean I–V relationship of amiloride‐sensitive currents was expressed by current density for wt (open circle, n = 15) and cpk (dark circle, n = 13) cells. Values are means ±SE, *p < .05.