FIG. 2.
(A) Competition EMSA experiments with the S1 probe. Radioactive S1 probe was incubated with CD4 SP D10 TH clone nuclear extracts alone (lane 2) or with 50- and 250-fold molar excess nonradioactive S1 (lanes 3 and 4), S2 (lanes 5 and 6), E box (lanes 7 and 8), and pKs linker (9 and 10). (B) Competition EMSAs with the S1 probe and MutB and MutC oligonucleotides. Radioactive S1 probe was incubated with CD4 SP D10 TH clone nuclear extracts alone (lane 2) or with 50- and 250-fold excess nonradioactive S1 (lanes 3 and 4), MutB (mB) (lanes 5 and 6), and MutC (mC) (lanes 7 and 8), or with pKs linker (lanes 9 and 10). (C) Competition EMSAs with the GST–HES-1 fusion protein. Competitions were conducted with 50- and 250-fold excess nonradioactive S1 (lanes 3 and 4), MutB (mB) (lanes 5 and 6), and MutC (mC) (lanes 7 and 8) or with pKs linker (lanes 9 and 10).