FIG. 3.
(A) EMSAs using the S1 probe and decreasing amounts of nuclear extracts from D10 (1, 0.5, 0.3, 0.1, and 0 μg), 293T cells transfected with CMV-HES-1 (1, 0.5, 0.3, and 0 μg), or mock-transfected 293T cells (1, 0.5, 0.3, and 0 μg). The major complex is indicated by the arrow. (B) Competition EMSAs using either D10 or CD8 SP L3 TC clone nuclear extracts alone or with either the N box (lanes 1 to 7) or the S1 probe (lanes 8 to 16). Competitor oligonucleotides were added at 50- and 250-fold excess.